Deleted
Sample Discontinued
Experience: None
Not Used
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Coding

Part:BBa_K351005:Design

Designed by: Dongchan Yang   Group: iGEM10_KAIST-Korea   (2010-07-22)


Normal Human FGF receptor with fission yeast surface signal peptide


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 610
    Illegal PstI site found at 175
    Illegal PstI site found at 1741
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 610
    Illegal NheI site found at 1392
    Illegal PstI site found at 175
    Illegal PstI site found at 1741
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 610
    Illegal BglII site found at 950
    Illegal BglII site found at 2099
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 610
    Illegal PstI site found at 175
    Illegal PstI site found at 1741
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 610
    Illegal PstI site found at 175
    Illegal PstI site found at 1741
    Illegal NgoMIV site found at 528
    Illegal NgoMIV site found at 1119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1401
    Illegal BsaI.rc site found at 1889
    Illegal BsaI.rc site found at 2253


Design Notes

This sequence contain signal peptide for fission yeast. You can't use this sequence directly for other organism like budding yeast.


Source

Signal petpdie is from Cell wall integrity and stress response component 1 of Fission yeast. Protein is from Normal human FGFR sequence withour signal peptide

References